Neil Smith National Wildlilfe Refuge Prairie Learning Center, Prairie City, Iowa
Why saline and YPD media is used for cryoprotectant?
May 30, 2010 by Aisling | Posted in Biology
Help!
They're not.
DMSO or glycerol are added to the saline or media specifically as cryoprotectants. At 10-20% concentrations they prevent the formation of ice crystals, which poke holes in the cell membranes, killing it. Regular media freezes and crystallizes.
The reason for saline or YPD is to provide an isotonic environment (and as for the YPD, it's because that's what cells are grown in and we're too lazy to change what they're suspended in when we freeze the cells down - just throw in the glycerol and freeze :P )
sponge | May 30, 2010
Egg Freezing | Fertility Preservation
then re-expands as the cryoprotectant enters the egg in a physiologically stable amount. This is critical for vitrification when highly ...
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Nation's Largest Fertility Center Helps More Patients Achieve Success with the In the traditional, slow freezing process, embryos would be exposed to multiple cryoprotectants, added in a specific order, over a 20 minute period. The cryoprotectant liquid would then be slowly cooled to -321 degrees Fahrenheit - the temperature of and more »
Interestingly, this stage of egg development may prove to be a more successful approach for cryopreservation because its oolemma is more permeable to cryoprotectant, and its chromatin is more conveniently and safely packaged in the nucleus (Van Blerkom
The caterpillars have evolved the ability to produce internal antifreeze, called a cryoprotectant, which allows their body temperature to go below freezing without suffering damage. I bet they prefer sheds and woodpiles over the meadow floor because and more »
Banded woolly bears also produce a cryoprotectant chemical that enables them to withstand freezing temperatures. One study in western Pennsylvania demonstrated this caterpillar could withstand –5C temperatures over a period of seven days with 100 per and more »
The true woolly bear, is the larval form of mature woolly bears who are in search for overwintering sites, inside bark or cavities of rocks or logs and it survives winter freezes by producing a cryoprotectant in its tissues. Although Curran's study was
