"It's 2011 and another year means getting older.. but aging may be a thing of the 'past' in the very near 'future' (it's a brain-teaser sentence I know :-)" ~Tomitheos
Copyright © 2011 Tomitheos Photography - All Rights Reserved
The 'freezing'...
you see, we are going to use it for our science project,and since we don't have nitrogen, can you please give us some substitute?
PULEZ?
dry ice is easily obtainable ~will not `cool` as much ,but the basic principle is still there.
put `dry ice` in your browser to find a local supply
you could then put the dry ice and seeds in a vacuum(thermos)flask
hope this helps
In meat storage, we do quick freezing and slow thawing to kill microorganisms. In cell culture, we store cell lines by slow freezing to protect the cells. However, we resuscitate cells by rapidly raising the temperature to 37 degrees celcius. How does this process help protect our cells?
I used to do cell cultures long time ago but I no longer, I'll do my best to answer. Freezing solution usually has DMSO which is toxic to the cells. While thawing, you do it quickly at 37 or between your fingers and replace the freezing solution with culture medium (has no DMSO) to prevent excessive amounts of DMSO to enter the cells.
i really need this answer!
cryopreservation occurs when cells are frozen with liquid nitrogen until it reaches a temperature of -100 degrees C. This makes the cell slow down. it even keeps it from dying because it is so cold. Scientists replace the expanding liquid with a substance sorta like anti-freeze. this keeps the cell from growing with the liquids. ex: whne water freezes the ice expands so thats kinda what happens in a cell it breaks.
its sorta like that :)
Thawing procedure for peripheral blood mononuclear cells (PBMC's) or cell lines cryopreserved in liquid nitrogen (LN2).
It is aimed to trigger the development of close cooperation between specialists from CEMIB and those from other institutions world wide, to train a group of biologists in cryopreservation and assisted reproduction techniques and to stimulate discussions to address the future needs and requirements of the Brazilian Scientific community in this area.”
|
BioLife Solutions Announces 7th Consecutive Quarter of Record Revenue BOTHELL, Wash., May 14, 2012 /PRNewswire via COMTEX/ -- BioLife Solutions, Inc. (OBB:BLFS) , a leading developer, manufacturer and marketer of proprietary clinical grade hypothermic storage and cryopreservation freeze media for cells and tissues, and more » |
How would-be grandparents are paying for adult daughters to freeze their eggs ...
For many would-be-grandparents, helping to cover the cost of the expensive oocyte cryopreservation is both an investment in their daughters' futures and in their own as well. But such an arrangement, no matter how fortunate, is often the result of an
|
|
Characterization of vascular endothelial progenitor cells from chicken bone marrow The viability of chicken EPCs, before and after cryopreservation was 92.2% and 81.1%, respectively. Thus, cryopreservation had no obvious effects on the viability of chicken EPCs. Dil-ac-LDL and FITC-UAE-1 uptake assays and immunofluorescent detection |
|
European Patent Office Revokes Controversial Patent The patent, EP1257168, was one of a family of closely related patents held in several countries by Inguran LLC (doing business as Sexing Technologies LLC and XY LLC) relating to the use of cryopreservation technology for sperm cells sorted into and more » |
|
Cryo-Cell International Taps Leader in Stem Cell Therapy to Serve as Chief ... Her expertise will ensure that we continue to exceed the industrys quality standards and maintain our tradition of offering clients the absolute best in cord blood, cord tissue, and menstrual stem cell cryopreservation services, said David Portnoy, and more » |